Primary fixation of vervet monkey oral epithelium for SEM and TEM

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dc.contributor.author Grossman, E. S.
dc.date.accessioned 2017-09-05T07:31:28Z
dc.date.available 2017-09-05T07:31:28Z
dc.date.issued 1987
dc.identifier.citation Grossman, E.S.1989. Primary fixation of vervet monkey oral epithelium for ultrastructural investigation‐SEM.Journal of Oral Pathology & Medicine;18(4):220-223. en_ZA
dc.identifier.uri http://hdl.handle.net/10539/23068
dc.description KIM en_ZA
dc.description.abstract Vervet monkey attached gingiva and alveolar mucosa was used to investigate the effect of primary fixative composition and osmolarity on the scanning electron microscope appearance and epithelial cell surface feature density. Primary fixation was obtained using 12 different fixatives with osmolarities varying between 320-2010mOsm followed by further standard SEM processing procedures. All primary fixatives investigated produced acceptably fixed oral epithelium for SEM study, showing all the morphologic features characteristic of either keratinized or non-keratinized oral tissue. Point counting revealed that the density of microvilli of attached gingiva epithelial cells when fixed at 2010mOsm was 72 +/- 8% of the cell surface area. This decreased to 40 +/- 5% when fixed at 320mOsm. Similarly the microplication density of the alveolar mucosa epithelial cells decreased from 70 +/- 5% at 2010mOsm to 43 +/- 7% at 320mOsm. Both these differences proved to be highly significant en_ZA
dc.language.iso en en_ZA
dc.subject Epithelium/ultrastructure en_ZA
dc.subject Mouth Mucosa/ultrastructure en_ZA
dc.subject Cercopithecus aethiops en_ZA
dc.title Primary fixation of vervet monkey oral epithelium for SEM and TEM en_ZA
dc.type Article en_ZA


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