Clinical, histological and microbiological study of hand-excavated carious dentine in extracted permanent teeth

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dc.contributor.author Bönecker, M.
dc.contributor.author Grossman, E.
dc.contributor.author Cleaton-Jones, P. E.
dc.contributor.author et al
dc.date.accessioned 2016-04-29T13:48:57Z
dc.date.available 2016-04-29T13:48:57Z
dc.date.issued 2003
dc.identifier.citation Bönecker,M., Grossman,E., Cleaton-Jones,P.E., et al. 2003. Clinical, histological and microbiological study of hand-excavated carious dentine in extracted permanent teeth. Journal of the South African dental association;58(7):273-278 en_ZA
dc.identifier.uri http://hdl.handle.net/10539/20317
dc.description KIM en_ZA
dc.description.abstract Changes in cultivable flora in dentine samples collected before and after hand excavation were examined in association with clinical status of the cavity surface, light microscopy and scanning electron microscopy (SEM). Thirty-five extracted permanent molar teeth with an occlusal caries lesion were excavated with hand instruments according to the atraumatic restorative treatment (ART) approach. Excavation pressure, dentine colour and consistency were recorded at the dentine-enamel junction (DEJ) prior to carious dentine removal and at the cavity floor after the final excavation; a microbiological sample of dentine was taken at both stages. Twelve restored teeth; six with positive and six with negative bacterial growth on the second sample, were selected for light microscopy and SEM. The hand-excavation removed tooth structure was soft, irreversibly damaged, dark and highly infected. Hand excavation reached dentine of increased hardness with a more normal colour to provide a sound structural base for restoration. Light and SEM examination of the cavity floor showed infected dentinal tubules in all 12 teeth examined. Linear logistic analysis showed a statistical association between light-yellow dentine on the cavity floor and an absence of bacterial growth (P = 0.006). This short-term in vitro study showed that caries-producing bacteria remained in dentine close to the cavity floor in 26/35 teeth despite clinical observations that indicated a suitably prepared cavity floor. en_ZA
dc.language.iso en en_ZA
dc.subject Dental Caries en_ZA
dc.subject Dentin en_ZA
dc.subject Molar/microbiology en_ZA
dc.title Clinical, histological and microbiological study of hand-excavated carious dentine in extracted permanent teeth en_ZA
dc.type Article en_ZA


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