Assessing Global Transcriptome Changes in Response to South African Cassava Mosaic Virus [ZA-99] Infection in Susceptible Arabidopsis thaliana.

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dc.contributor.author Pierce, E.J.
dc.contributor.author Rey, M.E.C.
dc.date.accessioned 2016-10-17T10:39:37Z
dc.date.available 2016-10-17T10:39:37Z
dc.date.issued 2013-06-27
dc.identifier.citation Pierce, E.J. and Rey, M.E.C. en_ZA
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/10539/21214
dc.description.abstract In susceptible plant hosts, co-evolution has favoured viral strategies to evade host defenses and utilize resources to their own benefit. The degree of manipulation of host gene expression is dependent on host-virus specificity and certain abiotic factors. In order to gain insight into global transcriptome changes for a geminivirus pathosystem, South African cassava mosaic virus [ZA:99] and Arabidopsis thaliana, 4×44K Agilent microarrays were adopted. After normalization, a log2 fold change filtering of data (p<0.05) identified 1,743 differentially expressed genes in apical leaf tissue. A significant increase in differential gene expression over time correlated with an increase in SACMV accumulation, as virus copies were 5-fold higher at 24 dpi and 6-fold higher at 36 dpi than at 14 dpi. Many altered transcripts were primarily involved in stress and defense responses, phytohormone signalling pathways, cellular transport, cell-cycle regulation, transcription, oxidation-reduction, and other metabolic processes. Only forty-one genes (2.3%) were shown to be continuously expressed across the infection period, indicating that the majority of genes were transient and unique to a particular time point during infection. A significant number of pathogen-responsive genes were suppressed during the late stages of pathogenesis, while during active systemic infection (14 to 24 dpi), there was an increase in up-regulated genes in several GO functional categories. An adaptive response was initiated to divert energy from growth-related processes to defense, leading to disruption of normal biological host processes. Similarities in cell-cycle regulation correlated between SACMV and Cabbage leaf curl virus (CaLCuV), but differences were also evident. Differences in gene expression between the two geminiviruses clearly demonstrated that, while some global transcriptome responses are generally common in plant virus infections, temporal host-specific interactions are required for successful geminivirus infection. To our knowledge this is the first geminivirus microarray study identifying global differentially expressed transcripts at 3 time points. en_ZA
dc.description.sponsorship Funding was from the National Bioinformatics Network through the Department of Science and Technology (www.dst.gov.za) and the National Research Foundation (www.nrf.ac.za). en_ZA
dc.language.iso en en_ZA
dc.publisher Public Library of Science. en_ZA
dc.rights © 2013 Pierce, Rey. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. en_ZA
dc.subject phytohormone en_ZA
dc.subject plant RNA en_ZA
dc.subject transcriptome en_ZA
dc.subject Arabidopsis thaliana en_ZA
dc.subject cell cycle regulation en_ZA
dc.subject cell transport en_ZA
dc.subject coevolution en_ZA
dc.subject evolutionary adaptation en_ZA
dc.subject Geminivirus en_ZA
dc.subject gene expression en_ZA
dc.subject gene repression en_ZA
dc.subject genetic transcription en_ZA
dc.subject host pathogen interaction en_ZA
dc.subject host range en_ZA
dc.subject immune evasion en_ZA
dc.subject microarray analysis en_ZA
dc.subject oxidation reduction reaction en_ZA
dc.subject signal transduction en_ZA
dc.subject South African cassava mosaic virus en_ZA
dc.subject South African cassava mosaic virus infection en_ZA
dc.subject viral plant disease en_ZA
dc.subject virus load en_ZA
dc.subject virus pathogenesis en_ZA
dc.title Assessing Global Transcriptome Changes in Response to South African Cassava Mosaic Virus [ZA-99] Infection in Susceptible Arabidopsis thaliana. en_ZA
dc.type Article en_ZA
dc.journal.volume 8 en_ZA
dc.journal.title PLoS ONE. en_ZA
dc.description.librarian NCS2016. en_ZA
dc.citation.doi 10.1371/journal.pone.0067534. en_ZA
dc.citation.issue 6 en_ZA


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